|
KMID : 0359320200600040195
|
|
Korean Journal of Veterinary Research
2020 Volume.60 No. 4 p.195 ~ p.202
|
|
|
Biological and molecular characterization of feline caliciviruses isolated from cats in South Korea
|
|
Yang Dong-Kun
Park Yu-Ri
Yoo Jae-Young
Choi Sung-Suk
Park Ye-Seul
An Sung-Jun
Park Jung-Won
Kim Heui-Jin
Kim Jong-Ho
Kim Ha-Hyun
Hyun Bang-Hun
|
|
|
Abstract
|
|
|
|
: Feline calicivirus (FCV) infection results in a common upper respiratory disease associated with oral ulceration in cats. Although FCV infection has been reported in cats worldwide, the biologic and genetic features of South Korean FCV are unclear. We aimed to investigate the biological and genetic features of South Korean FCV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FCV from 58 organ homogenate samples. The FCV isolates were confirmed by cytopathic effects, immunofluorescence, electron microscopy, and reverse transcription polymerase chain reaction assays. Viral genetic analysis was carried out with VP2 gene and complete genomes of FCVs. Five viruses propagated in CRFK cells were confirmed to be FCVs. The FCV17D283 isolate showed the highest viral titer of 107.2 TCID50/mL at 36 h post-inoculation. Korean FCV isolates did not grow well in Vero, BHK-21, A72, or Madin-Darby canine kidney cells. The FCV17D03 and FCV17D283 isolates had the highest genetic similarity (80.1% and 86.9%) with the UTCVM-H1 and 14Q315 strains, which were isolated in the United States and South Korea in 1995 and 2014, respectively. We isolated five FCVs from cats and detected important genetic differences among them. FCV isolates did not show any virulent effects in mice.
|
|
|
KEYWORD
|
|
|
feline calicivirus, diagnosis, phylogenetic analysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|